RAP1A antibody

Catalog No. ABIN969562

This Mouse Monoclonal antibody specifically detects RAP1A in ELISA and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for RAP1A antibody (ABIN969562)

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAP1A antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: 5F8G2

Product Details anti-RAP1A Antibody

Purpose: RAP1A Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human RAP1A expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-RAP1A antibody (ABIN969562)

Severson, Lee, Capaldo, Nusrat, Parkos: "Junctional adhesion molecule A interacts with Afadin and PDZ-GEF2 to activate Rap1A, regulate beta1 integrin levels, and enhance cell migration." in: Molecular biology of the cell, Vol. 20, Issue 7, pp. 1916-25, (2009) (PubMed).

Dao, Dupuy, Gavet, Caron, de Gunzburg: "Dynamic changes in Rap1 activity are required for cell retraction and spreading during mitosis." in: Journal of cell science, Vol. 122, Issue Pt 16, pp. 2996-3004, (2009) (PubMed).

Target Details for RAP1A

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Alternative Name: RAP1A

Background: The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50 % amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Molecular Weight: 43.2 kDa

UniProt: P62834

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor