PLK1 antibody (AA 331-508)

Catalog No. ABIN969580

This Mouse Monoclonal antibody specifically detects PLK1 in ELISA and ICC. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for PLK1 antibody (AA 331-508) (ABIN969580)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC)

Clone: 1D1

Product Details anti-PLK1 Antibody

Binding Specificity: AA 331-508

Purpose: PLK1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-PLK1 antibody (ABIN969580)

Lee, Rhee: "PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis." in: The Journal of cell biology, Vol. 195, Issue 7, pp. 1093-101, (2011) (PubMed).

Li, Zhou, Ye, Cheng, Zhou, Lu, Xie: "Reduced miR-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein." in: European journal of cancer (Oxford, England : 1990), Vol. 47, Issue 14, pp. 2166-74, (2011) (PubMed).

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: PLK1 is critical for the initiation of centrosome maturation. Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1, -2 and -3 are ubiquitously expressed, whereas PLK4 is restricted to a few tissues including the testes and the thymus. The mRNA and protein expression of PLK1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. Furthermore, PLK3 is a much more stable protein than PLK1, -2 or -4. PLK1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 overexpression correlates with cellular proliferation and poor prognosis. PLK2 and PLK3 are involved in checkpoint-mediated cell cycle arrest to ensure genetic stability. Loss-of-function mutations in these enzymes can lead to oncogenic transformation.

Molecular Weight: 68 kDa

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase