5-Methylcytosine antibody

Catalog No. ABIN969947

The Mouse Monoclonal anti- antibody has been validated for FACS, ICC, IP, ELISA, RIA, IHC (fp) and ChIP. It is suitable to detect in samples from . There are 15+ publications available.

Quick Overview for 5-Methylcytosine antibody (ABIN969947)

Target: 5-Methylcytosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Radioimmunoassay (RIA), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Chromatin Immunoprecipitation (ChIP)

Clone: 33D3

Product Details

Characteristics: This is mouse monoclonal antibody recognizes methylated CpG island. It was cited and validated on many applications, including: ELISA (0.1 μg/mL), Flow Cytometry (1 μg/mL), IHC of Cryo Sections (5-10 μg/mL), IHC of Paraffin Sections (5-10 μg/mL), and RIA (0.1 μg/mL).

Purification: Protein A ceramic hyper DF affinity chromatography

Purity: > 95 % (Gel electrophoresis SDS PAGE. Densitometry analysis)

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Ready for usage. For longer periods of storage, store at -20°C. Can be stored 1 month at 4°C.Avoid repeat freeze-thaw cycles.

References

Gertych, Oh, Wawrowsky, Weisenberger, Tajbakhsh: "3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models." in: BMC pharmacology & toxicology, Vol. 14, pp. 11, (2013) (PubMed).

Tajbakhsh: "Covisualization of methylcytosine, global DNA, and protein biomarkers for In Situ 3D DNA methylation phenotyping of stem cells." in: Methods in molecular biology (Clifton, N.J.), Vol. 1052, pp. 77-88, (2013) (PubMed).

Fukuda, Ichiyanagi, Yamada, Go, Udono, Wada, Maeda, Soejima, Saitou, Ito, Sasaki: "Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes." in: Journal of human genetics, Vol. 58, Issue 7, pp. 446-54, (2013) (PubMed).

Kurita, Arai, Nakamoto, Kato, Niwa: "Determination of DNA methylation using electrochemiluminescence with surface accumulable coreactant." in: Analytical chemistry, Vol. 84, Issue 4, pp. 1799-803, (2012) (PubMed).

Kurita, Niwa: "DNA methylation analysis triggered by bulge specific immuno-recognition." in: Analytical chemistry, Vol. 84, Issue 17, pp. 7533-8, (2012) (PubMed).

Toth, Maki: "A Commentary on the implications of the ENHANCE (Ezetimibe and Simvastatin in Hypercholesterolemia Enhances Atherosclerosis Regression) Trial: Should ezetimibe move to the "Back of the Line" as a therapy for dyslipidemia?" in: Journal of clinical lipidology, Vol. 2, Issue 5, pp. 313-7, (2011) (PubMed).

Dhawan, Georgia, Tschen, Fan, Bhushan: "Pancreatic ? cell identity is maintained by DNA methylation-mediated repression of Arx." in: Developmental cell, Vol. 20, Issue 4, pp. 419-29, (2011) (PubMed).

Macaulay, Weeks, Andrews, Morison: "Hypomethylation of functional retrotransposon-derived genes in the human placenta." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 22, Issue 11-12, pp. 722-35, (2011) (PubMed).

Walsh, Brisson, Robertson, Gordon, Jaubert-Possamai, Tagu, Edwards: "A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum." in: Insect molecular biology, Vol. 19 Suppl 2, pp. 215-28, (2010) (PubMed).

Minardi, Lucarini, Filosa, Milanese, Zizzi, Di Primio, Montironi, Muzzonigro: "Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens." in: Journal of cellular and molecular medicine, Vol. 13, Issue 8B, pp. 2115-21, (2010) (PubMed).

Zhang, Wang, Yu, Ming, Jiang: "DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya." in: Genome research, Vol. 18, Issue 12, pp. 1938-43, (2008) (PubMed).

Jensen, Novak, Eblin, Gandolfi, Futscher: "Epigenetic remodeling during arsenical-induced malignant transformation." in: Carcinogenesis, Vol. 29, Issue 8, pp. 1500-8, (2008) (PubMed).

Vrba, Junk, Novak, Futscher: "p53 induces distinct epigenetic states at its direct target promoters." in: BMC genomics, Vol. 9, pp. 486, (2008) (PubMed).

Zhang, Lee, Koo, Jiang: "Epigenetic modification of centromeric chromatin: hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize." in: The Plant cell, Vol. 20, Issue 1, pp. 25-34, (2008) (PubMed).

Maki, Mishra, Cameron, Filanoski, Rastogi, Maki: "Nanowire-transistor based ultra-sensitive DNA methylation detection." in: Biosensors & bioelectronics, Vol. 23, Issue 6, pp. 780-7, (2007) (PubMed).

Target Details

Target: 5-Methylcytosine

Alternative Name: 5-Methylcytosine (Methyl-CpG)

Target Type: Chemical

Background: Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90 % of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.