Cdc2, p34 (C-Term) antibody

Details for Product No. ABIN97117
Antigen
Epitope
C-Term
Reactivity
Human
1
1
Host
Rabbit
2
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
2
2
2
1
Options
Immunogen cdc2 peptide corresponding to the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Antigen
Background P34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF).  The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues.  In humans the phosphor-ylation appears to be performed by p60src.  The active form of the protein is dephosphorylated and it functions by phosphor-ylating a number of proteins.  The phosphorylation activity is coupled to the entry into the M-phase of the cell.  p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally.  Association with deletion mutants of cyclin halts the M-phase before it is completed.
Synonyms: Cell Divsion Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, DKFZp686L20222 antibody, MGC111195 antibody, p34 antibody, p34 Cdk1 antibody, p34 protein kinase antibody
Gene ID 983
UniProt P06493
Application Notes This antibody is suitable for immunoblotting, immunohistochemistry immuno-precipitation (as active kinase), and immunoblotting.  HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting.  LEP fibroblast cell lysate is suggested as a negative control. This product is suitable for the detection by immunoblot of human, rat and mouse cdc2. For immunohistochemistry use paraffin embedded tissue.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Cdc2, p34 (C-Term) antibody (ABIN97117) anti-cdc2 Cyclin Dependent Kinase was used to detect human p34cdc2by western blot in ...