Cyanine antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN97919

Supplier Product No.: 600-605-209

The Sheep Polyclonal anti-Cyanine antibody has been validated for ELISA, IHC, WB and DB. It is suitable to detect Cyanine in samples from .

Quick Overview for Cyanine antibody (Alkaline Phosphatase (AP)) (ABIN97919)

Target: Cyanine

Host: Sheep

Clonality: Polyclonal

Conjugate: This Cyanine antibody is conjugated to Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Product Details anti-Cyanine Antibody (Alkaline Phosphatase (AP))

Supplier Product No.: 600-605-209

Supplier: Rockland

Purpose: Cyanine Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum and cyanine conjugated IgG.

Characteristics: Synonyms: sheep anti-Cyanine Antibody alkaline phosphatase Conjugation, alk phos conjugated sheep anti-Cyanine Antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Cyanine Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and dot blot. This product has been assayed against 1.0 μg of Cyanine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:700 to 1:2,800 of the reconstitution concentration is suggested for this product. 

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.4 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details for Cyanine

Target: Cyanine

Target Type: Chemical

Background: Background: Cyanine is a synthetic family of dyes that can cover the spectrum from infrared to ultraviolent light. Cyanine is useful in protein and nucleic acid labeling. This Anti-Cyanine Antibody is conjugated to Alkaline Phosphatase. Anti-Cyanine (sheep) antibody is ideal for investigators in Cell Biology, Immunology, and Microbiology research.