Fluorescein antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN99341

Supplier Product No.: 600-105-096

Product Details

Target: Fluorescein

Host: Goat

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-105-096

Supplier: Rockland

Purpose: Fluorescein Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-Alkaline Phosphatase (calf intestine) and Fluorescein conjugated IgG.

Characteristics: Synonyms: goat Anti-FITC antibody alkaline phosphatase conjugation, alk phos conjugated goat anti-fluorescein antibody, alkaline phosphatase conjugation goat Anti-FITC antibody, alk phos conjugated goat anti-fluorescein antibody

Purification: Fluorescein Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Fluorescein conjugated to Goat IgG

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Fluorescein Antibody Alkaline Phosphatase Conjugated has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, in situ hybridization and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Background: Fluorescein Antibody Alkaline Phosphatase Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).