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Fluorescein antibody (HRP)

WB, ELISA, IHC, DB Host: Goat Polyclonal HRP
Rockland
Catalog No. ABIN99343
Supplier Product No.: 600-103-096
  • Target
    Fluorescein
    Host
    • 28
    • 18
    • 10
    • 8
    • 4
    • 2
    Goat
    Clonality
    • 29
    • 27
    • 14
    Polyclonal
    Conjugate
    • 45
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HRP
    Application
    • 32
    • 22
    • 18
    • 18
    • 17
    • 13
    • 11
    • 10
    • 8
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
    Supplier Product No.
    600-103-096
    Supplier
    Rockland
    Purpose
    Fluorescein Antibody Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum and Fluorescein conjugated IgG.
    Characteristics
    Synonyms: goat Anti-FITC antibody HRP conjugation, peroxidase conjugated goat anti-fluorescein antibody, peroxidase conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody HRP conjugation
    Purification
    Fluorescein Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen

    Immunogen: Fluorescein conjugated to Goat IgG

    Immunogen Type: Other

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: Anti-Fluorescein Antibody Peroxidase Conjugated has been tested by ELISA, dot blot, and western blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and in situ hybridization, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

    Western Blot Dilution: 1:5,000 - 1:10,000

    ELISA Dilution: 1:200,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Fluorescein
    Target Type
    Chemical
    Background
    Background: Anti-Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).
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