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Fluorescein antibody (Texas Red (TR))

The Goat Polyclonal anti- antibody has been validated for WB, FACS, FLISA and FM. It is suitable to detect in samples from .
Rockland
Catalog No. ABIN99347
Supplier Product No.: 600-109-096

Quick Overview for Fluorescein antibody (Texas Red (TR)) (ABIN99347)

Target

Fluorescein

Host

  • 28
  • 18
  • 10
  • 8
  • 4
  • 2
Goat

Clonality

  • 29
  • 27
  • 14
Polyclonal

Conjugate

  • 45
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Texas Red (TR)

Application

  • 32
  • 23
  • 18
  • 18
  • 17
  • 14
  • 10
  • 9
  • 8
  • 5
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
  • Supplier Product No.

    600-109-096

    Supplier

    Rockland

    Purpose

    Fluorescein Antibody Texas Red™ Conjugated

    Cross-Reactivity (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

    Characteristics

    Synonyms: goat Anti-FITC antibody Texas Red™ conjugation, Texas Red™ conjugated goat anti-fluorescein antibody, Texas Red™ conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody Texas Red™ conjugation

    Purification

    Fluorescein Antibody Texas Red™ Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Immunogen

    Immunogen: Fluorescein conjugated to Goat IgG

    Immunogen Type: Other

    Isotype

    IgG

    Labeling Ratio

    2.9
  • Application Notes

    Flow Cytometry Dilution: User Optimized

    Application Note: Anti-Fluorescein Antibody Texas Red™ Conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

    Western Blot Dilution: >1:10,000

    FLISA Dilution: >1:20,000

    IF Microscopy Dilution: 1:500 - 1:2,500

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Expiry Date

    12 months
  • Target

    Fluorescein

    Target Type

    Chemical

    Background

    Background: Fluorescein Antibody Texas Red™ Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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