GLN1 antibody

Catalog No. ABIN99838

Supplier Product No.: 100-101-238

This Goat Polyclonal antibody specifically detects GLN1 in WB, IP and ELISA. It exhibits reactivity toward Brevibacterium.

Quick Overview for GLN1 antibody (ABIN99838)

Target: GLN1 (Glutamine Synthetase (GLN1))

Reactivity: Brevibacterium

Host: Goat

Clonality: Polyclonal

Conjugate: This GLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-GLN1 Antibody

Supplier Product No.: 100-101-238

Supplier: Rockland

Purpose: Glutamine Synthetase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutamine Synthetase [Microbial].

Characteristics: Synonyms: Glutamine synthetase, goat anti-Glutamine Synthetase Antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Glutamine Synthetase [native from brevibacterium species]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Glutamine Synthetase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase

Background: Background: Catalytic activity of Glutamine Synthetase is ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Gene ID: 3345165

UniProt: Q79VE3

Pathways: Positive Regulation of Peptide Hormone Secretion