GPD1 antibody

Catalog No. ABIN99895

Supplier Product No.: 100-101-210

This Goat Polyclonal antibody specifically detects GPD1 in WB and ELISA. It exhibits reactivity toward Rabbit.

Quick Overview for GPD1 antibody (ABIN99895)

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This GPD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GPD1 Antibody

Supplier Product No.: 100-101-210

Supplier: Rockland

Purpose: Glycerol-3-Phosphate Dehydrogenase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle].

Characteristics: Synonyms: goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: This product has been assayed against 1.0 μg of Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GPD1

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Alternative Name: GPD1

Background: Background: Catalytic activity of Glycerol-3-Phosphate-Dehydrogenase is sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Gene ID: 100339469, 3043365

UniProt: P08507