Glycogen Synthase 1 antibody (pSer641)

Catalog No. ABIN99926

Supplier Product No.: 600-401-415

The Rabbit Polyclonal anti-Glycogen Synthase 1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect Glycogen Synthase 1 in samples from Human and Mouse.

Quick Overview for Glycogen Synthase 1 antibody (pSer641) (ABIN99926)

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glycogen Synthase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Glycogen Synthase 1 Antibody

Binding Specificity: pSer641

Supplier Product No.: 600-401-415

Supplier: Rockland

Purpose: Glycogen Synthase 1 phospho S641 Antibody

Cross-Reactivity (Details): Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase.

Characteristics: Synonyms: rabbit anti-muscle glycogen synthase pS641 antibody, rabbit anti-glycogen synthase pS641 antibody, Glycogen antibody, Glycogen synthase 1 (muscle) antibody, Glycogen synthase 1 antibody, Glycogen synthase1 antibody, GYS 1 antibody, GYS-1 antibody, GYS1 antibody, Starch synthase muscle antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Human Muscle Glycogen Synthase phospho peptide corresponding to a S641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This phospho specific polyclonal antibody was tested by immunoblotting, immunohistochemistry, and ELISA.  By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.   Immunoblotting will detect human and mouse muscle glycogen synthase.  Although not tested, this antibody is likely functional in immunoprecipitation.

Western Blot Dilution: 1:1,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:60,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Phospho GYS Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glycogen Synthase 1

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Alternative Name: GYS1

Background: Background: Anti-Glycogen synthase 1 pS641 is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units.  Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000.  GS is subject to regulation through both allosteric and covalent modification and occurs in two forms:  the phosphorylated inactive form, and the dephosphorylated active form.  GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase.  This antibody is specific for the phosphorylated form of GS at S641.   Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Gene ID: 2997

UniProt: P13807

Pathways: PI3K-Akt Signaling, AMPK Signaling, Cellular Glucan Metabolic Process