MODified Histone Peptide Array
PeA
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- Target
- Histone
- Application
- Peptide Array (PeA)
- Purpose
- The MODified Histone Peptide Array is a valuable research tool that can be used to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications (PTM).
- Brand
- MODified™
- Specificity
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MODified Histone Peptide Array Specifications:
- Standard microscope slides (26x76 mm, white coating)
- 768 spots per slide (384 peptide-conjugate spots printed in duplicate)
- Spot-to-spot distance 1.2 mm
- Peptides are covalently bound to cellulose via C-terminus
- Arrays contain control peptides and location marks - Characteristics
- The MODified Histone Peptide Array is a valuable research tool that can be used to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications (PTM). The MODified Histone Peptide Arrays screen 59 acetylation, methylation, phosphorylation, and citrullination modifications on the N-terminal tails of histones H2A, H2B, H3 and H4. Each peptide array contains 384 unique histone modification combinations in duplicate, including up to four separate modifications on the same 19mer peptide, offering the most extensive coverage available for commercial arrays of similar format. This extensive coverage of histone modifications enables the study of not only individual sites, but also the effects of neighboring modifications on recognition and binding. The unique synthesis and conjugation methods ensure greater than 95% purity of bound peptides and high peptide density at each spot, which is advantageous for analysis of interaction sites with low binding constants. The simple array protocol works like a Western blot; no special equipment needed.
- Components
- MODified Histone Peptide Array - peptide arrays contain 384 unique histone modification combinations. Arrays are available individually or in packs of 5 arrays.
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- Application Notes
- The MODified Histone Peptide Arrays can be used to screen antibodies, enzymes and proteins for cross reactivity or binding interactions with histones and their post-translational modifications. Following a blocking step, the array is incubated with either antibodies, proteins or enzymes for one hour, washed and then incubated for one hour with either a horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibody. Developing solution is then added and the image is captured using film or a CCD camera; no special equipment is needed. The MODified Histone Peptide Arrays can also be analyzed using the LI-COR® Odyssey® infrared imaging system and infrared detection reagents
- Comment
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MODified Histone Peptide Array - peptide arrays contain 384 unique histone modification combinations.
The MODified Array Labeling Kit (ABIN2866137) can be used to study antibody and enzyme interactions.
The MODified Protein Domain Binding Kit (ABIN 2866149) is designed to interrogate the binding specificity of His-tagged protein reading domains. - Restrictions
- For Research Use only
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- Target
- Histone
- Synonyms
- Histone H2A Array, Histone H2B 1 Array, Histone H3 Array, Probable histone H2B 4 Array, Histone H2B 2 Array, Histone Array, his-16 Array, his-12 Array, his-11 Array, his-9 Array, his-43 Array, his-61 Array, his-48 Array, his-57 Array, his-22 Array, his-4 Array, his-3 Array, his-42 Array, his-66 Array, his-65 Array, Histone Array
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