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Human Adhesion Molecule Array GS1

Reactivity: Human AA Fluorometric Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Glass Slide
Catalog No. ABIN4955950
  • Reactivity
    Human
    Detection Method
    Fluorometric
    Method Type
    Sandwich ELISA
    Application
    Antibody Array (AA)
    Purpose
    G-Series Human Adhesion Molecule Array 1 Kit. Semi-quantitative measurement of 17 human cell adhesion molecules. Suitable for all liquid sample types.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    ALCAM (CD166), BCAM, CEACAM-1, E-Cadherin, TROP1 (EpCAM), E-Selectin, ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), L-Selectin (CD62L), NCAM-1 (CD56), NrCAM, P-Cadherin, P-Selectin, PECAM-1 (CD31), VCAM-1 (CD106), VE-Cadherin (CDH5)
    Characteristics
    • High sensitivity (pg/mL to ng/mL levels)
    • High specificity and system reproducibility
    • Suitable for diverse sample types
    • Low sample volume requirement: 50 μL or less
    • Get results same day (6-hour processing time)
    • Well-suited for high throughput assays
    • Test 16 samples on each slide
    • Data analysis software provides one-step computation
    Components
    Cytokine Antibody Array glass slide (4 or 8 arrays per slide)
    Biotinylated Detection Antibodies
    Streptavidin-conjugated HiLytePlus™ 555 Fluor
    Blocking Buffer
    20X Wash Buffer I
    20X Wash Buffer II
    2X Cell Lysis Buffer
    G-Series Antibody Array accessories
    Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film
    Material not included
    Small plastic boxes or containers
    Pipettors, pipette tips and other common lab consumables
    Orbital shaker or oscillating rocker
    Aluminum foil
    Gene microarray scanner or similar laser fluorescence scanner
  • Application Notes
    Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70 μL of sample or reagent is used. Several incubation steps such as step 6 (blocking), step 7 (sample incubation), step 10 (detection antibody incubation), or step 13 (Cy3 equivalent dyestreptavidin incubation) may be done overnight at 4 °C. Please make sure to cover the incubation chamber tightly to prevent evaporation.
    Comment

    The G-Series arrays feature fluorescent signal detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection.
    All G-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.

    Sample Volume
    100 μL
    Plate
    Glass Slide
    Protocol
    1. Dry the glass slide
    2. Block array surface
    3. Incubate with Sample
    4. Incubate with Biotinylated Detection Antibody Cocktail
    5. Incubate with Streptavidin-Conjugated Fluor
    6. Disassemble the glass slide
    7. Scan with a gene microarray laser scanner
    8. Perform densitometry and analysis
    Restrictions
    For Research Use only
  • Handling Advice
    Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The G-Series slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with a fine point permanent marker. Please write the number on the very bottom edge of the slide, taking care to avoid writing on the array well areas.
    Storage
    -20 °C
    Storage Comment
    For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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