Mouse Cytokine Array Q4000

Catalog No. ABIN4956077

Product Details

Reactivity: Mouse

Detection Method: Fluorometric

Method Type: Sandwich ELISA

Application: Antibody Array (AA), Multiplex ELISA (mpELISA)

Purpose: Quantibody® Mouse Cytokine Array 4000 Kit. A combination of Mouse Cytokine Array Q4, Mouse Cytokine Array Q5, Mouse Cytokine Array Q6, Mouse Cytokine Array Q7, and Mouse Cytokine Array Q8. Detects 200 Mouse Cytokines. Suitable for all liquid sample types.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), Ace, Activin A, ADAMTS-1 (METH1), Adiponectin (ACRP30), ALK-1, Amphiregulin, Angiopoietin-3 (ANGPTL1), ANGPTL3, Artemin, Axl, BAFF R, Betacellulin (BTC), bFGF, BLC (CXCL13), C5a, Cardiotrophin-1 (CT-1), CCL28 (MEC), CCL6, CD27 (TNFRSF7), CD27 Ligand (TNFSF7), CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD32a, CD36 (SR-B3), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD48 (SLAMF2), CD6, CD80 (B7-1), Chemerin, Chordin, Clusterin, CRP (C-Reactive Protein), CTLA-4 (CD152), CXCL16, Cystatin C, DAN, Decorin, DKK-1, DLL4, Dtk, E-Cadherin, EDAR, EGF, Endocan, Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Epigen, Epiregulin, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fetuin A, Flt-3 Ligand, Fractalkine (CX3CL1), Galectin-1, Galectin-3, Galectin-7, Gas 1, Gas 6, GCSF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, gp130, Granzyme B, Gremlin-1, H60, HAI-1, HGF, HGFR, I-309 (TCA-3/CCL1), ICAM-1 (CD54), IFN-gamma, IFN-gamma R1, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R4 (ST2), IL-1 ra (IL-1 F3), IL-10, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-17 RB, IL-17A, IL-17B, IL-17E (IL-25), IL-17F, IL-2, IL-2 R alpha, IL-20, IL-21, IL-22, IL-23 p19, IL-28A (IFN-lambda 2), IL-3, IL-3 R beta, IL-33 (IL-1 F11), IL-4, IL-5, IL-6, IL-7, IL-7 R alpha, IL-9, I-TAC (CXCL11), JAM-A (CD321/F11R), KC (CXCL1), Kremen-1, Leptin, Leptin R, Limitin, Lipocalin-2 (NGAL), LIX, LOX-1, L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), MAdCAM-1, Marapsin, MBL-2, MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), Meteorin, MFG-E8, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 gamma, MIP-2, MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-10, MMP-2, MMP-3, Neprilysin, Nope, NOV (CCN3), Osteoactivin (GPNMB), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 Ligand, P-Cadherin, PDGF-AA, Pentraxin-3 (TSG-14), Periostin, Persephin, Platelet Factor 4 (CXCL4), PlGF-2, Progranulin, Prolactin, Pro-MMP-9, Prostasin, P-Selectin, RAGE, RANTES (CCL5), Renin 1, Resistin, SCF, SDF-1 alpha (CXCL12 alpha), sFRP-3, SLAM (CD150), Sonic Hedgehog N-Terminal (Shh-N), TACI (TNFRSF13B), TARC (CCL17), TCK-1 (CXCL7), TECK (CCL25), Testican 3, TGF beta 1, Thrombopoietin (TPO), TIM-1 (KIM-1), TNF alpha, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL (TNFSF10), Trance (TNFSF11), TREM-1, TREML1 (TLT-1), TROY (TNFRSF19), Tryptase epsilon, TSLP, TWEAK (TNFSF12), TWEAK R (TNFRSF12), VCAM-1 (CD106), VEGF-A, VEGF-B, VEGF-D, VEGFR1, VEGFR2, VEGFR3

Characteristics:

• Running an array is like running dozens of ELISAs simultaneously.
• Quantibody arrays are stunningly simple to use, read, and analyze.
• Each panel can quantify up to 40 different biomarkers simultaneously, and individual panels can be multiplexed to quantify as many as 660 different biomarkers at one time.
• The entire process can be completed in just 4-6 hours.
• More cost-effective than traditional ELISA
• High specificity and system reproducibility
• Suitable for diverse sample types
• Low sample volume requirement: 50 μL or less
• Well-suited for high throughput assays

• More cost-effective than traditional ELISA
• High specificity and system reproducibility
• Suitable for diverse sample types
• Low sample volume requirement: 50 μL or less
• Get results same day (6-hour processing time)
• Well-suited for high throughput assays
• Q Analyzer software provides one-step computation

Components: Glass Chip with antibody arrays
Sample Diluent
Lyophilized protein standard mix
Detection antibody cocktail
Streptavidin-Fluorescent dye
Wash buffer

Material not included: Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

Application Details

Application Notes: Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70 μL of sample or reagent is used. Several incubation steps such as step 6 (blocking), step 7 (sample incubation), step 10 (detection antibody incubation), or step 13 (Cy3 equivalent dyestreptavidin incubation) may be done overnight at 4 °C. Please make sure to cover the incubation chamber tightly to prevent evaporation.

Comment:

The Quantibody arrays are quantitative multiplex ELISA arrays featuring fluorescent detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection. Cytokine standards are provided with the array for calculation of target protein concentrations.
All Quantibody arrays feature the sandwich immunoassay principle, utilizing an immobilized capture antibody along with a corresponding biotinylated detection antibody.

Sample Volume: 100 μL

Plate: Glass Slide

Protocol:

1. Each Quantibody array starts with a single glass microscope slide, which acts as a support for the array. Slides are segmented using a rubber gasket. Up to 8 samples may assayed using a single slide.
2. Antibodies against a variety of different antigens (up to 40 biomarkers per slide) are printed onto the glass slide. Replicates are included, saving you both time and precious sample volume.
3. The end-user adds either known concentration standards (included) or aqueous sample to each well on the slide. Antibodies on the slide capture antigen off from the sample or standard.
4. The end-user adds a detection mix containing paired antibodies (compatible with the primaries pre-coated on the slide) conjugated to a fluorescent dye for detection.
5. Fluorescent signal from each spot is read using a laser slide scanner. The intensity from each spot is compared to the standard curve, and a quantitative expression profile for relevant biomarkers is established.

Restrictions: For Research Use only

Handling

Handling Advice: Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The Quantibody slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with an ultra-fine point permanent marker. Please Note:Red permanent marker can significantly interfere with fluorescent signal detection. We recommend marking your slides with a green, blue or black ultra-fine point permanent marker. Please write the number on the very bottom edge of the slide. Do not write on the arrayed well areas.

Storage: -20 °C

Storage Comment: For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

References

Erikson, Valente, Mummidi, Kandikattu, DeMarco, Bender, Fay, Siebenlist, Chandrasekar: "Targeting TRAF3IP2 by Genetic and Interventional Approaches Inhibits Ischemia/Reperfusion-induced Myocardial Injury and Adverse Remodeling." in: The Journal of biological chemistry, Vol. 292, Issue 6, pp. 2345-2358, (2017) (PubMed).

Schnepp, Lee, Guldner, OTighearnaigh, Howe, Palakurthi, Eckert, Toni, Ashfeld, Zhang: "GAD1 Upregulation Programs Aggressive Features of Cancer Cell Metabolism in the Brain Metastatic Microenvironment." in: Cancer research, Vol. 77, Issue 11, pp. 2844-2856, (2017) (PubMed).

Yariswamy, Yoshida, Valente, Kandikattu, Sakamuri, Siddesha, Sukhanov, Saifudeen, Ma, Siebenlist, Gardner, Chandrasekar: "Cardiac-restricted Overexpression of TRAF3 Interacting Protein 2 (TRAF3IP2) Results in Spontaneous Development of Myocardial Hypertrophy, Fibrosis, and Dysfunction." in: The Journal of biological chemistry, Vol. 291, Issue 37, pp. 19425-36, (2016) (PubMed).

Kurtz, Elkins: "Correlates of Vaccine-Induced Protection against Mycobacterium tuberculosis Revealed in Comparative Analyses of Lymphocyte Populations." in: Clinical and vaccine immunology : CVI, Vol. 22, Issue 10, pp. 1096-108, (2015) (PubMed).