Human Glycosylation Array 507

Catalog No. ABIN4973902

Quick Overview for Human Glycosylation Array 507 (ABIN4973902)

Reactivity: Human

Detection Method: Fluorometric

Application: Antibody Array (AA)

Sample Type: Plasma, Cell Culture Supernatant, Serum, Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Product Details

Purpose: Human Glycosylation Antibody Array 507. For the simultaneous detection of the glycosylation protein profile of 507 human proteins in serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

Specificity: 6Ckine, Activin A, Activin B, Activin C, Activin RIA (ALK-2), Activin RIB (ALK-4), Activin RII A/B, Activin RIIA, Adiponectin, AgRP, ALCAM, Angiogenin, Angiopoietin 1, Angiopoietin 2, Angiopoietin-4, ANGPTL1, ANGPTL2, Angiopoietin-like Factor, Angiostatin, APJ, APRIL, Amphiregulin, Artemin, Axl, B7-1 (CD80), BACE-1, BAFF R (TNFRSF13C), bax, BCMA (TNFRSF17), BD-1, BDNF, beta-Catenin, b-NGF, BIK, BLC (BCA-1, CXCL13), BMP-15, BMP-2, BMP-3, BMP-3b (GDF-10), BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BMPR-II, BTC, Cardiotrophin-1 (CT-1), CCL14 (HCC-1, HCC-3), CCL28 (VIC), CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD 163, CD14, CD27 (TNFRSF7), CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5, CD154), Cerberus 1, Chem R23, Chordin-Like 1, Chordin-Like 2, CLC, CNTF, CNTF R alpha, Coagulation Factor III (Tissue Factor), Coagulation Factor X/Xa, CRIM 1, Cripto-1, Crossveinless-2 (CV-2), CRTH-2, Cryptic, Csk, CTACK (CCL27), CTGF (CCN2), CTLA-4 (CD152), CXCL14 (BRAK), CXCL16, CXCR1 (IL-8 RA), CXCR2 (IL-8 RB), CXCR3, CXCR4 (fusin), CXCR5 (BLR-1), CXCR6, D6, DAN, DANCE, DcR3 (TNFRSF6B), Decorin, Dkk-1, Dkk-3, Dkk-4, DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, EDA-A2, EDAR, EDG-1, EGF, EGFR (ErbB1), EG-VEGF (PK1), EMAP-II, ENA-78, Endocan, Endoglin (CD105), Endostatin, Endothelin, EN-RAGE, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2), Eotaxin-3 (CCL26), Epiregulin, ErbB2, ErbB3, ErbB4, Erythropoietin, E-Selectin, FADD, FAM3B, Fas (TNFRSF6), Fas Ligand, bFGF, FGF R3, FGF R4, FGF R5, FGF-10, FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-8, FGF-9, FGF-BP, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Galectin-3, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), G-CSF, G-CSF R (CD 114), GDF1, GDF11, GDF15, GDF3, GDF5, GDF8, GDF9, GDNF, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR (TNFRF18), GITR Ligand (TNFSF18), GLO-1, Glucagon, Glut1, Glut2, Glut3, Glut5, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, Granzyme A, Grb2, GREMLIN, GRO, GRO alpha, Growth Hormone, Growth Hormone R, HB-EGF, HCC-4 (CCL16), HCR (CRAM-A/B), Hepassocin, HGF, HGFR, HRG-alpha, HRG-beta 1, HVEM (TNFRSF14), I-309, ICAM-1, ICAM-2, ICAM-3 (CD50), ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-gammabeta, IFN-gamma, IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-rp1 (IGFBP-7), IGF-1, IGF-1 sR, IGF-2, IGF-2 R, IL-1 alpha, IL-1 beta, IL-1 F10 (IL-1HY2), IL-1 F5 (FIL1delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 R3 (IL-1 R AcP), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 R8, IL-1 R9, IL-1 ra, IL-1 sRI, IL-1 sRII, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 BPa, IL-18 R alpha (IL-1 R5), IL-18 R beta (AcPL), IL-19, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 BP, IL-22 R, IL-23, IL-23 R, IL-24, IL-26, IL-27, IL-28A, IL-29, IL-3, IL-3 R alpha, IL-31, IL-31 RA, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, Insulin, Insulin R, Insulysin (IDE), IP-10, I-TAC (CXCL11), KGF-2, Kininostatin (Kininogen), Kremen-1, Kremen-2, Latent TGF beta bp1, LBP, Lck, LECT2, Lefty - A, Leptin (OB), Leptin R, LFA-1 alpha, LIF, LIF R alpha, Light, Lipocalin-1, Lipocalin-2, LRP-1, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), Lymphotoxin beta (TNFSF3), Lymphotoxin beta R (TNFRSF3), MAC-1, MCP-1, MCP-2, MCP-3, MCP-4 (CCL13), M-CSF, M-CSF R, MDC, MFG-E8, MFRP, MIF, MIG, MIP 2, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-10, MMP-11 (Stromelysin-3), MMP-12, MMP-13, MMP-14, MMP-15, MMP-16 (MT3-MMP), MMP-19, MMP-2, MMP-20, MMP-24 (MT5-MMP), MMP-25 (MT6-MMP), MMP-3, MMP-7, MMP-8, MMP-9, MSP alpha chain, Musk, NAP-2, NCAM-1 (CD56), Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGF R, NOV (CCN3), NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin A, Orexin B, OSM, Osteoactivin (GPNMB), Osteocrin, Osteoprotegerin (Osteoprotegerin), OX40 Ligand (TNFSF4), PARC (CCL18), PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PECAM-1 (CD31), Pentraxin3 (TSG-14), Peroxiredoxin 6 (Prdx6), Persephin, PF4 (CXCL4), PLGF, PLUNC, Pref-1, Progranulin, Prolactin, P-selectin, RAGE, RANK (TNFRSF11A), RANTES, RELM beta, RELT (TNFRSF19L), ROBO4, S100 A8/A9, S100A10, SAA, SCF, SCF R (CD117), SDF-1 (CXCL12), sFRP-1, sFRP-3, sFRP-4, sgp130, SIGIRR, Siglec-5 (CD170), Siglec-9, SLPI, Smad1, Smad4, Smad5, Smad7, Smad8, Soggy-1, Sonic Hedgehog (ShhN-terminal), SPARC, Spinesin, TACI (TNFRSF13B), Tarc, TCCR (WSX-1), TECK (CCL25), TFPI, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta 5, TGF beta RI (ALK-5), TGF beta RII, TGF beta RIII, Thrombopoietin (TPO), Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin, Thyroid Peroxidase (TPX), Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A (TNFSF15), TLR1, TLR2, TLR3, TLR4, TMEFF1 (Tomoregulin-1), TMEFF2, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TNF alpha, TNF beta, TNFSF14, TRADD, TRAIL (TNFSF10), TRAIL R1 (DR4, TNFRSF10A), TRAIL R2 (DR5, TNFRSF10B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRANCE, TREM-1, TROY (TNFRSF19), TSG-6, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), Ubiquitin+1, uPA, uPAR, Vasorin, VCAM-1 (CD106), VE-Cadherin, VEGF-A, VEGFR2 (KDR), VEGFR3, VEGF-B, VEGF-C, VEGF-D, VEGI (TNFSF15), WIF-1, WISP-1 (CCN4), XEDAR

Characteristics:

• High density screening
• High sensitivity and specificity
• Low sample volume (20-100 μL per array)
• Large dynamic range of detection
• Compatible with most sample types
• Accurate and reproducible

Components: Human Glycosylation Array Glass Slide/s
Dilution Buffer
20X Wash Buffer I
20X Wash Buffer II
Cy3 equivalent dye-conjugated Streptavidin
Biotin-labeled Lectin Mixture
Adhesive device sealer
Manual

Material not included: Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Distilled or de-ionized water
1 mL microcentrifuge tubes
Gene microarray scanner or similar laser fluorescence scanner

Application Details

Comment:

Glycome Arrays allow researchers to efficiently obtain glycosylation profiles in their samples. Capture antibodies are printed onto glass slides and the glycans on these capture antibodies are removed. The glass slide arrays come pre-blocked and are ready to be incubated with samples. After incubation with samples and washing to remove unbound proteins, five unique biotin-labeled lectins are incubated with the array to allow binding to the glycans from captured target proteins. Streptavidin-conjugated fluorescent dye (Cy3 equivalent) is then added to the array. Finally, the glass slide is dried and laser fluorescence scanning is used to visualize the signals. These signals are then compared to the array map to identify glycosylated proteins present in the samples.

Sample Volume: 50 μL

Plate: Glass Slide

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, all components of the Human Glycosylation Array 507 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at -20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.