Protein G Sepharose

Catalog No. ABIN1607932

Supplier Product No.: pg50-00-0002

Quick Overview for Protein G Sepharose (ABIN1607932)

Target: Protein G

Application: Purification (Purif), Immunoprecipitation (IP)

Bead Ligand: Protein G

Bead Matrix: Sepharose beads

Bead Size: 90 µm

Product Details

Reactivity: Streptococcus

Supplier Product No.: pg50-00-0002

Supplier: Rockland

Purpose: Protein G is used for immunoprecipitation and purification of monoclonal and polyclonal antibodies.

Specificity: Sepharose™ Protein G binds to all IgG subclasses from human, monkey, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.

Characteristics: Protein G is prepared by covalently coupling recombinant Protein G to 6% crosslinked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG, resulting in IgG binding greater than 10 mg of human IgG per cc of slurry. Protein G is used for immunoprecipitation and purification of monoclonal and polyclonal antibodies. This product shows stability within pH range of 2 to 10, is high flow rate capable, and has low falling off of rProtein G.

Application Details

Application Notes: Protein G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in 20% Ethanol/Water

Storage: 4 °C

Storage Comment: Store SEPHAROSE PROTEIN G at 4 °C prior to opening. DO NOT FREEZE.

Expiry Date: 12 months

Target Details

Target: Protein G