(5 references)
Catalog No. ABIN1379872
Quick Overview for Fix Buffer I (ABIN1379872)
Application
Intracellular Flow Cytometry (ICFC)
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Brand
- BD Phosflow™
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Characteristics
- The BD Phosflow Fix Buffer I can be used for simultaneous fixation and permeabilization of cells prior to intracellular staining. The product contains 250 ml of a 1X buffer solution.
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS Buffer pH 7.5BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C
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Storage Comment
- Store undiluted at 4°C.
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: "Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 55, Issue 2, pp. 61-70, (2003) (PubMed).
: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells." in: European journal of immunology, Vol. 24, Issue 5, pp. 1097-101, (1994) (PubMed).
: "Detection of intracellular cytokines by flow cytometry." in: Journal of immunological methods, Vol. 159, Issue 1-2, pp. 197-207, (1993) (PubMed).
: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).
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: "Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 55, Issue 2, pp. 61-70, (2003) (PubMed).
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