Perm Buffer II
ICFC
Catalog No. ABIN1379875
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Quick Overview for Perm Buffer II (ABIN1379875)
Application
Intracellular Flow Cytometry (ICFC)
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Brand
- BD Phosflow™
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Characteristics
- BD™ Phosflow Perm Buffer II is intended to be used for the permeabilization of fixed PBMC's or lysed whole blood. PBMC's, for example, can be stimulated with kinase activators, such as phorbol esters, and can be fixed with BD™ Phosflow Fix Buffer I (Cat. No. 557870) followed by permeabilization of the cells with BD™ Phosflow Perm Buffer II to allow for an effective intracellular staining of kinases. Designed for optimal fluorescent staining in flow cytometry, BD™ Phosflow Perm Buffer II can be used with antibodies for phospho-protein analysis (i.e the staining of intracellular proteins involved in cell signalling pathways, such as phosphorylated proteins and assorted kinases). In many cases, these phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g CD markers). Not all cell surface markers, however, are compatible with BD™ Phosflow Perm Buffer II.
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
Blotto Blocking Reagent Immunoanalytical Grade (Non-Fat Dry Milk)Analytical Grade BCA, ELISA, FM, IP, WB 1 X
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Aqueous buffered solution containing proprietary ingredients.
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Storage
- 4 °C
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Storage Comment
- Store undiluted at room temperature.
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