PI/RNase Staining Buffer
FACS
Catalog No. ABIN3071643
Quick Overview for PI/RNase Staining Buffer (ABIN3071643)
Application
Flow Cytometry (FACS)
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Characteristics
- Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule with a molecular mass of 668.4 Da that can be used to stain cells. When excited with 488 nm wavelength light, it fluoresces red. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The content of DNA as determined by flow cytometry, can reveal useful information about the cell cycle and the proteins involved in the regulation of the cell cycle.
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- PBS (pH7.2), 0.09% NaN3
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Do not dilute or boil.
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