Loading Buffer (5X)
Catalog No. ABIN5564173
Quick Overview for Loading Buffer (5X) (ABIN5564173)
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Characteristics
- Loading buffer for separation and visualization proteins with SDS-PAGE and Western Blot analysis.
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS Buffer pH 7.5BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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Application Notes
- Sample can be prepared under reducing conditions with the addition of a reducing agent such as beta-mercaptoethanol or DTT. Dilute to 1X in sample. Do not heat prior to loading on gel. For reducing buffer, add 5 % (v/v) beta-mercaptoethanol or 1 mM DTT to 1X loading buffer and sample. Boil sample/loading buffer for 5 min. Do not store buffer with reducing agent, add fresh prior to each use.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- -20 °C
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Storage Comment
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Short Term Storage: +20°C
Long Term Storage: +20°C
Stable for at least 2 years after receipt when stored at +20°C. -
Expiry Date
- 24 months
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