10X RIPA Lysis Buffer

Catalog No. ABIN5706373

Supplier Product No.: mb-077-0015

Quick Overview for 10X RIPA Lysis Buffer (ABIN5706373)

Application: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Supplier Product No.: mb-077-0015

Supplier: Rockland

Purpose: 10X RIPA Lysis Buffer

Purification: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: This product is 10X concentrated stock solution. Dilute to 1X prior to use. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and

Western Blot Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 X

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT

Storage Comment: Store container at room temperature (18° to 26° C) prior to opening. Protect from light (store in the dark). Expiration date is six (6) months from date of receipt.

Expiry Date: 6 months

Target Details

Background:

Synonyms: 10X RIPA Lysis Buffer, RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer

Background: RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinit