Cell Lysis Buffer

Catalog No. ABIN5706385

Supplier Product No.: mb-078-0015

Quick Overview for Cell Lysis Buffer (ABIN5706385)

Application: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Supplier Product No.: mb-078-0015

Supplier: Rockland

Purpose: Cell Lysis Buffer

Purification: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.

ChIP Dilution: User Optimized

Western Blot Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 X

Storage: 4 °C,-20 °C

Storage Comment: Store cell lysis buffer at -20° C prior to opening. Buffer is stable for up to 2 years stored at -20°C. It is recommended to aliquot and freeze buffer for extended usage. Store thawed buffer at 2-8°C for no longer than 2 weeks. This product MAY be stable for up to two (2) weeks if properly stored and handled.

Expiry Date: 2 weeks

Target Details

Background:

Synonyms: Cell Lysis Buffer

Background: Cell Lysis Buffer is used to lyse cells under non-denaturing conditions. If recommended add 1 mM PMSF or other protease inhibitor immediately before use. Keep all reagents and lysates in ice. For adherent cells add 400μL of buffer per 10cm flask once