Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TMB Membrane Peroxidase Substrate - Ready-To-Use

IHC, WB
Catalog No. ABIN5706416
  • Application
    Immunohistochemistry (IHC), Western Blotting (WB)
  • Application Notes

    Immunohistochemistry Dilution: 1X

    Application Note: TMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB Membrane Peroxidase Substrate comes ready to use.

    Western Blot Dilution: 1X

    Comment

    Synonyms: TMB Membrane Peroxidase Substrate, TMB ELISA Peroxidase Substrate

    Background: TMB Membrane Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction of TMB can be halted with acid to change the TMB to yellow. TMB Membrane Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is ideal for investigators in Immunology, Cancer, and Microbiology research.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Stabilizer: Proprietary buffer with enhancer

    Storage
    4 °C
    Expiry Date
    24 months
You are here:
Support