Blocking Buffer (2X) for Fluorescent Western Blotting

Catalog No. ABIN6953293

Supplier Product No.: mb-070-0050

Quick Overview for Blocking Buffer (2X) for Fluorescent Western Blotting (ABIN6953293)

Application: Cellular Assay (CA), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)

Product Details

Supplier Product No.: mb-070-0050

Supplier: Rockland

Purpose: Blocking Buffer (2X) for Fluorescent Western Blotting

Purification: Blocking buffer is specifically formulated to achieve superior reproducible Western blotting images using this system.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: This product is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This buffer was meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI)

Western Blot Dilution: User Defined

Other: Blocking solution can be diluted 1:1 in TBS or used undiluted.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 X

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE.

Storage: 4 °C

Storage Comment: Store blocking buffer at 4° C prior to opening. DO NOT FREEZE. Expiration date is six (6) months from date of receipt.

Expiry Date: 6 months

Target Details

Background:

Synonyms: Multiplex Blocking Buffer, Fluorescent Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, IRDye Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer

Background: This blocking buffer is ideal for infrared Western blotting. Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophor