Quick Overview for Blotto B Blocking Reagent Pre-Mixed (ABIN925608)
Application
-
-
Supplier Product No.
- b553-0500
-
Supplier
- Rockland
-
Purpose
- Blotto B Pre-Mixed
-
Purification
- This product contains highly purified immunoanalytical grade non-fat dry milk and bovine serum albumin (protease and immunoglobulin-free BSA) dissolved in a solution of Tris buffered saline.
-
-
Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
-
-
Application Notes
-
Application Note: Use Rockland's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5 % non-fat dry milk and 0.05 % Tween-20) and BLOTTO B (solution of TBS, 1 % non-fat dry milk, 1 % BSA
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- Restore with deionized water (or equivalent)
-
Concentration
- 1 X
-
Handling Advice
- After reconstitution use blocking buffers immediately.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Working solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen. Contains TBS, 1% non-fat dry milk Expiration date is one (1) year from date of receipt.
-
Expiry Date
- 12 months
-
-
-
Background
-
Synonyms: Blotto B Pre-Mixed, TBS, 1 % non-fat dry milk, 1 % BSA, 0.05 % TWEEN-20, blocking reagents for western or ELISA
Background: Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of prot
-
-
