Catalog No. ABIN925629
Supplier Product No.: tmbe-100
Quick Overview for TMB ELISA Peroxidase Substrate (ABIN925629)
Application
ELISA
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Supplier Product No.
- tmbe-100
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Supplier
- Rockland
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Characteristics
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TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow.
Synonyms: 3,3’,5,5’-Tetramethylbenzidine; TMB-E; TMBE
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS pH 7.210 X
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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Application Notes
- TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 X
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Storage
- 4 °C
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