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Calpain 1+2 antibody

CAPN1+CAPN2 Reactivity: Cow, Human EIA, IP, WB Host: Mouse Monoclonal 28F3 unconjugated
Catalog No. ABIN187528
  • Target
    Calpain 1+2 (CAPN1+CAPN2)
    Reactivity
    Cow, Human
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    Species Reactivity: Reacts with human and bovine calpain, but does not recognize rat or mouse calpain.
    Immunogen
    Balb/C mice were immunized with calpain purified from human placenta. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.
    Clone
    28F3
    Isotype
    IgG1
  • Application Notes
    ELISA: Use at 0.5-1 µg/ml. Immunoprecipitation: Use at 1-2 µg/ml. Western blot: 0.5-1 µg/ml The optimal dilution for a specific application should be determined by the researcher.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Monoclonal antibody against human Calpain I, II (small subunit). This antibody is available at a concentration of 100 µg/100 µl in 0.02 M sodum phosphate, pH 7.5, 0.15 M sodium chloride, 50% glycerol, 3 mM sodium azide. This antibody was purified to >95
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Target
    Calpain 1+2 (CAPN1+CAPN2)
    Alternative Name
    Calpain (CAPN1+CAPN2)
    Background
    The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and u-calpain (calpain I) are composed of an 80 kDa subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. Whereas m-calpain requires milli-molar levels of calcium, u-calpain is active at micromolar concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. Secificity: Clone 28F3 specifically recognizes the 28-30 kDa subunit of m-calpain and µ-calpain.
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