ELISA, Western Blotting (WB), Radioimmunoassay (RIA)
Purpose
Mouse monoclonal antibody raised against Chondroitin-4-Sulfate.
Specificity
Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. There is no reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. Cross-reactivity with mouse, human, bovine, sheep, dog and rat.
Cross-Reactivity
Cow, Dog, Human, Mouse, Rat, Sheep
Immunogen
Native purified from mouse proteoglycan.
Clone
BE-123
Isotype
IgG1 kappa
Application Notes
Western Blot (1:1000-1:4000) ELISA (1:400-1:3200) Radioimmunoassay (1:50-1:6400) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In ascites
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.