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Antibody Conjugation

antibodies-online is together with our partner Rockland Immunochemicals, Inc. is ready to deliver - whether you need milligrams or grams of conjugated antibody. With decades of combined experience providing protein and antibody conjugation services, as well as producing ready-to-use conjugated proteins and antibodies, we advance a wide range of projects for the research, pharmaceutical, diagnostics, and biotech communities.

Our Antibody Conjugation Capabilities

Our antibody conjugation services team can create the ideal reagent for your immunofluorescence assay by conjugating your antibody or any of our ready-to-use antibodies to a wide range of classic fluorescent markers and next generation fluorochrome dyes that are designed for multiplex detection.

  • Peroxidase (HRP)
  • Biotin Alkaline Phosphatase (ALP)
  • Glucose Oxidase (GO)
  • β-Galactosidase Fluorescent dyes
  • ATTO Dye
  • Fluorescein: DyLight™, Phycoerythrins, Rhodamine, Texas Red

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Antibody Conjugation: Emission Spectra of Conjugates
Peroxidase Conjugation

Peroxidase Conjugation

We typically perform custom HRP conjugation on up to 20 mg of affinity-, Protein A-, or Protein G purified antibody. The peroxidase is conjugated to antibody through an added -CHO group, and typically yields a ratio of 2:1..

Rhodamine Conjugation

Supply up to 20 mg of antibody and we will conjugate to TRITC through amine groups and purify resulting in a F/P ratio of 1 to 5.

Rhodamine Conjugation
Biotin Conjugation

Biotin Conjugation

Conducted on up to 20 mg of antibody, we use a long arm spacer biotin derivative to conjugate biotin to your antibody, with a target purification of 10-15:1.