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HCP Antibodies

Benefit from the combined expertise of antibodies-online and our partner Rockland Immunochemicals, Inc. (Rockland). Leverage Rockland’s 15+ years of success generating custom polyclonal host cell protein antibodies (HCP antibodies) to keep your biopharmaceutical development program meeting deadlines and moving efficiently.

Our comprehensive, fully customizable services cover all aspects of anti-HCP antibody generation, from antigen preparation and assay development to documentation and manufacturing, with an end-goal of creating a broad immune response as measured by a high percent of coverage on a 2D Western blot.

Whether you need support from start-to-finish or are only outsourcing small parts of the project, you can be confident when you partner with us.

Highest quality and efficiency standards

Our HCP Antibody service combines expertise with optimized processes to maximize quality, coverage, and speed.

  • Consistent—Minimize lot-to-lot variability and ensure long term supply chain with well-managed antisera pools
  • High-quality—Maximize repeatability and safeguard quality with our rigorous testing and multi-validation studies
  • Scalable—Experience stable large-scale manufacturing with our strong internal processes
  • Transparent—Maintain visibility into your project with all development and manufacturing conducted at our US-based facility
  • Experienced—From initial intake to manufacturing and shipping, our experienced scientists, project managers, and logistics team ensure accuracy and efficiency

Start Your Project

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HCP Antibody Generation Timeline (Months)
1 2 3 4 5 6 7 8
Setup
Immunization & Boost
Reagent Generation
Sampling & Analysis
Reagent Qualification

Our Service Partner: Rockland

Our Service Partner: Rockland

Benefit from expertise over two generations of scientific advances. Rockland enabling biomolecule detection, characterization and analysis since 1962. 60,000 square feet manufacturing facility produce high-quality antibodies, reagents and custom services for your next project

Benefit from expertise over two generations of scientific advances. Rockland enabling biomolecule detection, characterization and analysis since 1962. 60,000 square feet manufacturing facility produce high-quality antibodies, reagents and custom services for your next project

Fully customize your anti-HCP antibody generation service

Choose all-inclusive anti-HCP antibody generation or select only the services you need.


Antigen Preparation

  • Product Platform: E. Coli, Yeast Insect, Plant, & Mammalian
  • Fractionation
  • Ultrafiltration
  • Crosslinking & Conjugation

Antisera Generation

  • Standard Protocol
  • Size Fractionated Immunogen
  • Cascade Immunogen
  • Rabbit, Goat, Chicken, Sheep

Reagent Characterization & Analysis

  • ELISA
  • 1D SDS & Western Blot
  • 2D SDS & Western Blot
  • Reagent Qualification
  • Coverage Analysis & Characterization

Assay Development

  • Scouting Study
  • Bridging Study
  • Process Specific HCP Assays
  • Multiple Platform Support
HCP-1D-SDS-PAGE-Western Blotting

See the strong, uniform signal of a final HCP antibody reagent

We use 1D SDS-PAGE followed by Western blot analysis on test and production bleeds to evaluate the host antibody-mediated immune response to HCP lysate.

We also evaluate HCP antibodies with 2D PAGE and Western blotting

We can also conduct 2D SDS-PAGE followed by Western blotting. 2D separation can deliver more complete insight into an anti-HCP antibody’s coverage of proteins in the host cell lysate than 1D SDS-PAGE and Western blotting

HCP-2D-SDS-PAGE-Western Blotting
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