EXOS antibody (cleaved)
EXOS
Reactivity: Pseudomonas aeruginosa
WB
Host: Chicken
Polyclonal
unconjugated
(1 reference)-
- Target
- EXOS (ExoS Gene Product (EXOS))
- Binding Specificity
- cleaved
- Reactivity
- Pseudomonas aeruginosa
- Host
-
Chicken
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- Pseudomonas aeruginosa only
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 48 kDa
- Purification
- affinity purified
- Immunogen
- amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
- Isotype
- IgY
-
- Application Notes
- 1: 5000 (WB)
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- PBS pH 8.0+ 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock. - Storage
- 4 °C
- Storage Comment
- store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
-
-
: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).
-
: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).
-
- Target
- EXOS (ExoS Gene Product (EXOS))
- Alternative Name
- Exoenzyme S (ExoS)
- Background
- Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.
- Molecular Weight
- 48 kDa
- UniProt
- Q51449
-
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