HSP18.5 antibody
Quick Overview for HSP18.5 antibody (ABIN619791)
Target
Reactivity
Host
Clonality
Application
-
-
Purpose
- Anti-HSP18,5 | class IV heat shock protein
-
Cross-Reactivity (Details)
-
Arabidopsis thaliana, Hordeum vulgare
Not reactive in: Prosopis cineraria -
Predicted Reactivity
- Glycne max,Medicago truncatula,Medicago sativa,Pisum sativum,Ricinus communis,Rosa chinensis,Zea mays
-
Purification
- Serum
-
Immunogen
- Recombinat Arabidopsis thaliana HSP18.5
-
-
-
Application Notes
- 1 : 1000 (WB)
-
Comment
-
hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- For reconstitution add 200 μL of sterile water
-
Buffer
- Serum
-
Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
-
Storage
- -20 °C
-
Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
-
-
- HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
-
Alternative Name
- HSP18.5
-
Background
-
Background: HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family and is involved in protein complex oligomerization and protein folding. Alternative names: AtHsp18.5, 18.5 kDa heat shock protein.
Additional information: As hsp18,5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature, Normally I heat stress the seedling on a sealed agar plate for 2 hours, This assures that the humidity around the plant is very high, Low humidity can allow the plant to cool down through transpiration, If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment, Heat stressing plants in microphage tubes does not work that well
-
Molecular Weight
- 18.5 kDa
-
Gene ID
- 816448
-
UniProt
- O64564
Target
-
