Influenza A H1N1 PB1 Protein (AA 283-486) (His tag)
This Recombinant Influenza A H1N1 PB1 protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN7937685
$2,902.48
Plus shipping costs $50.00
1 mg
Shipping to:
United States
Delivery in 23 to 40 Business Days
Quick Overview for Influenza A H1N1 PB1 Protein (AA 283-486) (His tag) (ABIN7937685)
Target
Influenza A H1N1 PB1 (PB1)
(Influenza Polymerase Basic 1 (PB1))
Protein Type
Recombinant
Origin
Influenza A Virus
Source
Escherichia coli (E. coli)
Purity
Greater than 85 % as determined by SDS-PAGE.
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Protein Characteristics
- AA 283-486
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Purification tag / Conjugate
- This Influenza A H1N1 PB1 protein is labelled with His tag.
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Purpose
- Recombinant Influenza A virus PB1 protein, partial
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Sequence
- ANVVRKMMTN SQDTELSFTI TGDNTKWNEN QNPRMFLAMI TYMTRNQPEW FRNVLSIAPI MFSNKMARLG KGYMFESKSM KLRTQIPAEM LASIDLKYFN DSTRKKIEKI RPLLIEGTAS LSPGMMMGMF NMLSTVLGVS ILNLGQKRYT KTTYWWDGLQ SSDDFALIVN APNHEGIQAG VDRFYRTCKL LGINMSKKKS YINR
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Comment
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C-terminal 6xHis-tagged
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Restrictions
- For Research Use only
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Format
- Lyophilized or Liquid
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Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50 % of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50 %. Customers could use it as reference.
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Buffer
- If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5 % -50 % glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6 % Trehalose, pH 8.0.
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Handling Advice
- Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
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Expiry Date
- 6-12 months
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- Influenza A H1N1 PB1 (PB1) (Influenza Polymerase Basic 1 (PB1))
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Alternative Name
- PB1
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Target Type
- Influenza Protein
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Background
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Relevance: RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs
Alternative Name(s): Polymerase basic protein 1,RNA-directed RNA polymerase subunit P1
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Molecular Weight
- 30.4 kDa
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UniProt
- P03431
Target
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