Influenza Polymerase Basic 2 Protein (PB2) (AA 318-484) (His tag)
Quick Overview for Influenza Polymerase Basic 2 Protein (PB2) (AA 318-484) (His tag) (ABIN7937686)
Target
Protein Type
Origin
Source
Purity
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Protein Characteristics
- AA 318-484
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Purification tag / Conjugate
- This Influenza Polymerase Basic 2 protein is labelled with His tag.
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Purpose
- Recombinant Influenza A virus PB2 protein, partial
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Sequence
- RISSSFSFGG FTFKRTSGSS VKREEEVLTG NLQTLKIRVH EGYEEFTMVG RRATAILRKA TRRLIQLIVS GRDEQSIAEA IIVAMVFSQE DCMIKAVRGD LNFVNRANQR LNPMHQLLRH FQKDAKVLFQ NWGVEPIDNV MGMIGILPDM TPSIEMSMRG VRISKMG
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Comment
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C-terminal 6xHis-tagged
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Restrictions
- For Research Use only
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Format
- Lyophilized or Liquid
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Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50 % of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50 %. Customers could use it as reference.
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Buffer
- If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5 % -50 % glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6 % Trehalose, pH 8.0.
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Handling Advice
- Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
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Expiry Date
- 6-12 months
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- Influenza Polymerase Basic 2 (PB2)
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Alternative Name
- PB2
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Target Type
- Influenza Protein
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Background
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Relevance: Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS
Alternative Name(s): RNA-directed RNA polymerase subunit P3
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Molecular Weight
- 25.8 kDa
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UniProt
- P03428
Target
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