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Z-IETD-FMK (Caspase-8 Inhibitor)

Name: Z-IETD-FMK (Caspase-8 Inhibitor)
SKU: ABIN2691060
Availability: OutOfStock

Func, CC, BR, InhA Cell-permeable Z-IETD-fluoromethylketone

(3 references)

Catalog No. ABIN2691060

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Quick Overview for Z-IETD-FMK (Caspase-8 Inhibitor) (ABIN2691060)

Application

Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)

Chemical Name

Z-IETD-fluoromethylketone

Permeability

Cell-permeable

Purpose

A caspase-8 inhibitor

Sequence

Z-Ile-Glu(OMe)-Thr-Asp(OMe)-FMK

Characteristics

Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-IETD-FMK is an irreversible and cell permeable inhibitor of caspase-8. The peptide is O-methylated in the P1 position on aspartic acid providing enhanced stability and increased cell permeability. This inhibitor can be used to inhibit caspase-8 activity and to study events downstream of caspase-8 activation. Z-IETD-FMK has a molecular weight of 654 Daltons. Flow cytometric analysis of Jurkat cells (Human T-cell leukemia, ATCC TIB-152). Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 μM Z-IETD-FMK (upper center and bottom center panels) or 20 μM of a negative control inhibitor Z-FA-FMK (upper right and bottom right panels) for 30 minutes, and then either left untreated (bottom row) or treated with 4 μM of campthothecin for 3 hours (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in camthothecin treated cells, approximately 42 % of the cells were induced to undergo apoptosis and the use of the caspase-8 inhibitor Z-IETD-FMK reduced the level of apoptosis to that observed in untreated controls. Cells treated with Z-FA-FMK (Cat. No. 550411) showed similar results to the treated cells without inhibitor, indicating that the control inhibitor did not attentuate apoptosis.

Purification

Purified

Formula

C₃₀H₄₃FN₄O₁₁

Solubility

DMSO
Geldanamycin- FITC
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Geldanamycin
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Radicicol
Inh 12772-57-5

Geldanamycin- Biotin
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Herbimycin A
Inh 70563-58-5 [(2R,3S,5S,6R,7S,8E,10S,11S,12E,14E)-2,5,6,11-tetramethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

17-GMB-APA-GA
Inh [(3R,5S,6R,7S,10S,11S)-21-[3-[4-(2,5-dioxopyrrol-1-yl)butanoylamino]propylamino]-6-hydroxy-5,11-dimethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

TEMPO
Inh 2564-83-2 2,2,6,6-Tetramethylpiperidine 1-oxyl, 2,2,6,6-Tetramethyl-1-piperidinyloxy, free radical, (2,2,6,6-Tetramethyl-1-piperidinyl)oxidanyl, 1,1,5,5-tetramethylpentamethylene nitroxide, 2,2,6,6-Tetramethylpiperidine-1-oxyl

Triacsin C
Inh 76896-80-5 (3E)-1-Oxo-3-[(2E,4E,7E)-2,4,7-undecatrien-1-ylidene]triazane

TPCK
Inh 402-71-1 N-(4-Chloro-3-oxo-1-phenyl-2-butanyl)-4-methylbenzenesulfonamide

Staurosporine
Inh 62996-74-1 (2S,3R,4R,6R)-3-Methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-16-one

Comment

BD Pharmingen™ Z-IETD-FMK, Caspase-8 Inhibitor – Purified
Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-IETD-FMK is an irreversible and cell permeable inhibitor of caspase-8. The peptide is O-methylated in the P1 position on aspartic acid providing enhanced stability and increased cell permeability. This inhibitor can be used to inhibit caspase-8 activity and to study events downstream of caspase-8 activation. Z-IETD-FMK has a molecular weight of 654 Daltons.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitute the Z-IETD-FMK inhibitor in DMSO before use. The reconstituted Z-IETD-FMK inhibitor may be stored in small aliquots at -20 °C.

Buffer

Lyophilized in dimethyl sulfoxide (DMSO).

Storage

-20 °C

Storage Comment

Avoid multiple freeze-thaws of product. Store the lyophilized Z-IETD-FMK inhibitor at -20°C. Reconstitute the Z-IETD-FMK inhibitor in DMSO before use. The reconstituted Z-IETD-FMK inhibitor may be stored in small aliquots at -20°C.
Wang, Zhen, Klug, Wood, Wu, Mizrahi: "Involvement of caspase 3- and 8-like proteases in ceramide-induced apoptosis of cardiomyocytes." in: Journal of cardiac failure, Vol. 6, Issue 3, pp. 243-9, (2001) (PubMed).

Gregoli, Bondurant: "Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors." in: Journal of cellular physiology, Vol. 178, Issue 2, pp. 133-43, (1999) (PubMed).

Thornberry, Lazebnik: "Caspases: enemies within." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1312-6, (1998) (PubMed).
Molecular Weight

654 Da