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Iqca1p1 Protein (AA 1-825) (Strep Tag)

This Recombinant Iqca1p1 protein is produced in Cell-free protein synthesis (CFPS).
Catalog No. ABIN3130910
starts at
$20,480.57
Plus shipping costs $50.00, if applicable $20.00 dry ice
250 μg
Shipping to: United States
Delivery in 55 to 67 Business Days

Quick Overview for Iqca1p1 Protein (AA 1-825) (Strep Tag) (ABIN3130910)

Target

Iqca1p1 (IQCA1P1) (IQ Motif Containing with AAA Domain 1 Pseudogene 1 (IQCA1P1))

Protein Type

Recombinant

Origin

Mouse

Source

Cell-free protein synthesis (CFPS)

Application

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade

custom-made
  • Protein Characteristics

    AA 1-825

    Purification tag / Conjugate

    This Iqca1p1 protein is labelled with Strep Tag.

    Sequence

    MSEGTYQRLW EASHVTLEEV LEKEPSVLEP VPSRERQSFQ YRISVLYLYY LGLLRRFNLA YDQMVQPQKR RLLRRLLDGV AGRVLELKDE LVRVDLCETH CLDRVLQDLK LTPADLEVPI PKYFQLEQSS AVKARQQMLA DILNRLEPLV SQENLRGLSR TAALILVQSA ERARQGRLRA TFMREIRKEE ERDRRIWENG QQKFSRDQGA IVIQKVWKGY LQRKRIEQDR RVEMEFIGML PSPNQTARLN TLNQAFLGEE SRRMRQVEKE EEFQEAIGKT YESLTETEGP DMKERMKDQI RQWFIECHAL TGRFPDYPDE ASGGSYLIFA DKTPEQVRLD LEAQAQESRK KDQEKKEKNK EKEKEKKEKK KKKVKEEKVK KEPEVMFRVL PSKSIPVINA GHEEYTSVWK SRYDNKHPSQ NFDSETLREE KRKQVEMEIR VQVDELMRQE LKNLRLAVDR EETRPLKSPK KKGGKKSGKK KKEKDLTPDR SVDSLFEELV IIGLIKKSAL VTLSDYIGDC LYLGSTLTLA NKMPMPSLFD IRQNMALYGV LRLGSHDIHT MAPLVRSILL VGPSGMGKKM LVQAVCTETG ANLFDLSPDN LMGKYPGKNG AQLLVHIVFK VARLLQPSVI WIGNTEKTFY KKVPKEERTM DPKRIKKDLM RATKQLSPGD RVMLIGTTER PQLAEMKGLC RFYERILFIP RPDYASRYVL WKRMIESQGR GVQLTSSLDV SALARVSDGY TPSHILQSIQ SVLTERRLLQ LIKKPLVASE FVGHLARLDP VYREEEESLK EWFFKTPLGK KNMKFTKDQL EAEEARLTKE KKKKK
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    Characteristics

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • Application Notes

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -80 °C

    Storage Comment

    Store at -80°C.

    Expiry Date

    12 months
  • Target

    Iqca1p1 (IQCA1P1) (IQ Motif Containing with AAA Domain 1 Pseudogene 1 (IQCA1P1))

    Alternative Name

    Iqca1l

    Background

    IQ and AAA domain-containing protein 1-like (Protein IQCA1P1)

    Molecular Weight

    96.0 kDa

    UniProt

    A6H690
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