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- Target See all IgE products
- IgE
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Host
- Mouse
- Application
- Isotype Control (IsoC), ELISA
- Characteristics
- The C38-2 antibody is specific for the hapten trinitrophenyl (TNP). The immunogen, TNP-KLH, is not expressed on human, mouse, or rat cells. In the absence of specific binding, this antibody may bind non-specifically to Fc receptors. The immunoglobulin from clone C38-2 was selected as an isotype control following screening for low background on a variety of mouse and human tissues. This antibody is routinely tested by ELISA. Other applications were tested during antibody development only or reported in the literature. IgE standard curve obtained using purified mAb R35-72, Cat. No. 553413, at 2 μg/mL for capture and biotinylated mAb R35-118, Cat. No. 553419, at 2 μg/mL for detection of the mouse IgE standard, mAb C38-2, Cat. No. 557079, following the enclosed "Mouse IgE ELISA Protocol".
- Purification
- Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc.
- Immunogen
- TNP-keyhole limpet hemocyanin
- Isotype
- IgE
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4° C.
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- Target
- IgE
- Abstract
- IgE Products
- Synonyms
- IGH2 Isotype Control, IgE Isotype Control, Gm900 Isotype Control, immunoglobulin heavy chain (epsilon polypeptide) Isotype Control, immunoglobulin heavy constant epsilon Isotype Control, Immunoglobulin heavy constant epsilon Isotype Control, Ighe Isotype Control, IGHE Isotype Control
- Background
- Synonyms: anti-TNP
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