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Rat IgG1 Isotype Control

FACS, IsoC, ELISA, IHC (fro), IHC (zinc), IHC (f) IgG1 unconjugated
Catalog No. ABIN2688750
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Quick Overview for Rat IgG1 Isotype Control (ABIN2688750)

Target

IgG1

Application

Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Isotype

IgG1
  • Host

    • 236
    • 84
    • 65
    • 14
    • 6
    • 5
    • 5
    • 2
    • 1
    • 1
    Rat

    Characteristics

    Following immunization of a rat with mouse immunoglobulin (Ig), the Ig from the R3-34 hybridoma was identified as a non-reactive clone. The R3-34 immunoglobulin was selected as an Ig isotype control following screening for low background staining on a variety of mouse and human cells and tissues.

    Purification

    Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc.

    Immunogen

    Mouse immunoglobulin
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Aqueous buffered solution containing ≤ 0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store undiluted at 4°C.
  • Hsu, Raine, Fanger: "Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 29, Issue 4, pp. 577-80, (1981) (PubMed).

  • Target

    IgG1

    Target Type

    Antibody

    Background

    Synonyms: IgG1, kappa, Ighg1, Igkc
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