Llama IgG3 isotype control (Biotin)
Quick Overview for Llama IgG3 isotype control (Biotin) (ABIN5608801)
Target
Conjugate
Application
Isotype
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Host
- Llama
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Supplier Product No.
- 025-0643
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Supplier
- Rockland
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Purpose
- Llama IgG3 Isotype Control Biotin Conjugated
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Characteristics
- Llama IgG3 isotype, Llama IgG3 subclass isotype, Llama IgG3 Biotin Conjugated, Llama IgG3 BAC, Llama isotype control
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Purification
- Llama IgG3 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. No bands corresponding to unconjugated llama IgG1 or IgG2 are observed. Biotin Conjugated Llama IgG3 was proven by Dot Blot.
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Mouse IgG3 isotype control
FACS, IsoC, ELISA, IHC Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype control (APC)FACS, ELISA Monoclonal B10 IgG3 APC
Mouse IgG3 isotype control (PE)FACS, ELISA Monoclonal B10 IgG3 PE
Mouse IgG3 isotype control (SPRD)FACS, ELISA Monoclonal B10 IgG3 SPRD
Mouse IgG3 isotype control (FITC)FACS, ELISA Monoclonal B10 IgG3 FITC
Mouse IgG3 isotype control (Biotin)FACS, ELISA Monoclonal B10 IgG3 Biotin
Mouse IgG3 isotype controlFACS, ELISA Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype control (FITC)IsoC, IF IgG3 FITC
Mouse IgG3 isotype controlFACS, IsoC, ELISA, WB, ICC, IP, IHC (fro), IHC (p) Monoclonal PPV-07 IgG3 unconjugated
Mouse IgG3 isotype control (PE)FACS, IsoC Monoclonal PPV-07 IgG3 PE
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Application Notes
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Flow Cytometry Dilution: User Optimized
Application Note: Llama IgG3 Biotin conjugated isotype control can be utilized as a control or standard reagent in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgG3
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Target Type
- Antibody
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Background
- Background: Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35 % - 70 % of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies.
Target
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