Quick Overview for Mouse IgG Isotype Control (ABIN5633244)
Target
Application
Isotype
-
-
Host
- Mouse
-
Supplier Product No.
- 010-0102-0005
-
Supplier
- Rockland
-
Purpose
- Mouse IgG Whole Molecule
-
Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
-
Characteristics
- Immunoglobulin G from Mouse, IgG from Mouse, purified polyclonal IgG antibody
-
Purification
- Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
-
-
Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
-
-
Application Notes
-
Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: User Optimized
Application Note: Mouse IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Western Blot Dilution: User Optimized
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
-
Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 500 μL
-
Concentration
- 10.0 mg/mL
-
Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store purified Mouse IgG at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
Expiry Date
- 12 months
-
-
- IgG
-
Target Type
- Antibody
-
Background
- Background: Mouse IgG purified protein (Immunoglobulin G) are antibody molecules. Mouse IgG is composed of four peptide chains - two heavy chains and two light chains. Mouse IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Mouse IgG typically constitutes 75 % of serum immunoglobulins. Mouse IgG molecules are synthesized and secreted by plasma B cells. Ideal as a negative control for Flow Cytometry, Western blotting, immunoprecipitation and immunohistochemistry applications.
Target
-
