Quick Overview for Rat IgG Isotype Control (ABIN5633248)
Target
Fragment
Application
Isotype
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Host
- Rat
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Supplier Product No.
- 012-0104-0002
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Supplier
- Rockland
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Purpose
- Rat IgG F(ab')2 Fragment
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat IgG F(ab')2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
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Characteristics
- Rat IgG F(ab')2 fragment, Rat IgG Fab2 fragment
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Purification
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Sterility
- Sterile filtered
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Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
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Application Notes
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Application Note: Rat IgG F(ab')2 Fragment has been tested in SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 2.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- IgG
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Target Type
- Antibody
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Background
- Background: Rat IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Rat IgG F(ab')2 Molecules lack the Fc portion of Rat IgG and therefore receptors that bind Rat IgG Fc will not bind Rat IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Target
-
