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Human IgM Isotype Control

ELISA, WB, IHC IgM unconjugated
Catalog No. ABIN5633257
  • Target See all IgM products
    IgM
    Host
    • 52
    • 36
    • 34
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Application
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Cross-Reactivity (Details)
    Human IgM (myeloma) whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgM (μ chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross-reactivity will occur with anti-Human IgG.
    Purification
    Human IgM (myeloma) whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
    Sterility
    Sterile filtered
    Isotype
    IgM
  • Application Notes

    Immunohistochemistry Dilution: User Optimized

    Application Note: Human IgM (myeloma) whole molecule can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.1 mg/mL
    Buffer

    Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target
    IgM
    Abstract
    IgM Products
    Synonyms
    IgM constant region Isotype Control, IGM Isotype Control
    Target Type
    Antibody
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