Human IgM Isotype Control
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- Target See all IgM products
- IgM
- Fragment
- Fab fragment
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Host
- Human
- Application
- ELISA, Western Blotting (WB)
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum. No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
- Purification
- HUMAN IgM (myeloma) Fab μ fragment was prepared from serum by a multi-step process which includes delipidation, selective precipitation, tandem molecular sieve chromatography and trypsin digestion followed by extensive dialysis against the buffer stated above.
- Sterility
- Sterile filtered
- Isotype
- IgM
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Mouse IgM isotype control
IsoC Monoclonal MML IgM unconjugated
Rat IgM isotype controlIsoC, ELISA, WB IgM Native unconjugated
Mouse IgM isotype controlIsoC Polyclonal IgM unconjugated
Mouse IgM isotype control (FITC)IsoC, FACS, IF IgM FITC
Human IgM isotype controlELISA, WB, IHC IgM unconjugated
Mouse IgM isotype controlIsoC, FACS IgM unconjugated
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype control (PE)IsoC, FACS, IF IgM PE
Rat IgM isotype control (FITC)IsoC, ELISA, WB, IM IgM Native FITC
Mouse IgM isotype control (APC)IsoC, FACS, IF IgM APC
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- Application Notes
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Application Note: Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. They can be used for these purposes because each Fab fragment has only a single antigen binding site. HUMAN IgM (myeloma) Fab μ fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target
- IgM
- Abstract
- IgM Products
- Synonyms
- IgM constant region Isotype Control, IGM Isotype Control
- Target Type
- Antibody
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