Human IgG Isotype Control
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Host
- Human
- Application
- Isotype Control (IsoC), Blocking Reagent (BR)
- Characteristics
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Purified Human IgG Fab'2 for use as a control or blocking reagent
Source: Normal Human Serum/Plasma was obtained from healthy donors of US origin.
Alternative Names: Human Immunoglobulin G Fab'2 - Purification
- Purified
- Purity
- > 95 % pure
- Sterility
- 0.2 μm filtered
- Isotype
- IgG
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Rabbit IgG isotype control
IsoC, ELISA, WB IgG Native unconjugated
Mouse IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlELISA, WB, IHC IgG unconjugated
Rabbit IgG isotype control (PE)ELISA, FACS Polyclonal IgG PE
Goat IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Rabbit IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rat IgG isotype controlELISA, WB IgG unconjugated
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Clear, colorless liquid
Country of Origin: Normal Human Serum/Plasma was obtained from healthy donors of US origin. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride and 0.05 % (w/v) Sodium Azide. Protease/IgG free.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Fab'2 fragments of antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact some of the hinge region. Fab'2 fragments have two antigen-binding Fab portions linked together by disulfide bonds, and therefore are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of antibodies to live cells with Fc receptors or to Protein A or Protein G.
Synonyms: Human Immunoglobulin G Fab'2. - Molecular Weight
- 110 kDa
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