(1 reference)-
- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Host
- Hamster
- Conjugate
- FITC
- Application
- Isotype Control (IsoC), Immunofluorescence (IF)
- Characteristics
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Purified Hamster IgG (Fab'2) FITC conjugate
Alternative Names: Hamster Immunoglobulin G (Fab'2) (FITC) - Purification
- Horse IgG (FITC) was purified by delipidation, salt fractionation, ion exchange chromatography followed by dialysis.
- Isotype
- IgG
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Rabbit IgG isotype control
IsoC, ELISA, WB IgG Native unconjugated
Mouse IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlELISA, WB, IHC IgG unconjugated
Rabbit IgG isotype control (PE)ELISA, FACS Polyclonal IgG PE
Goat IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Rabbit IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rat IgG isotype controlELISA, WB IgG unconjugated
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized from 0.01 M Na1O4, pH 7.4, with 0.11 NaCl, 10 mg/mL BSA, and 0.01 % thimerisol. Immunoglobulin and protease free.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
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Product is photosensitive and should be protected from light.
Avoid repeated freeze/thaw cycles. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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: "Naturally-existing CD4(+)CD25(+)Foxp3(+) regulatory T cells are required for tolerance to experimental autoimmune thyroiditis induced by either exogenous or endogenous autoantigen." in: Journal of autoimmunity, Vol. 33, Issue 1, pp. 68-76, (2009) (PubMed).
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: "Naturally-existing CD4(+)CD25(+)Foxp3(+) regulatory T cells are required for tolerance to experimental autoimmune thyroiditis induced by either exogenous or endogenous autoantigen." in: Journal of autoimmunity, Vol. 33, Issue 1, pp. 68-76, (2009) (PubMed).
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope - the antigen-binding site - at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. Treatment with pepsin produces an F(ab)'2 fragment.
Synonyms: Hamster Immunoglobulin G (Fab'2) (FITC).
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