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Mouse IgG2b Isotype Control

FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal PLRV219 IgG2b unconjugated
Catalog No. ABIN93873

Quick Overview for Mouse IgG2b Isotype Control (ABIN93873)

Target

IgG2b

Clonality

  • 121
  • 2
  • 1
Monoclonal

Application

Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

  • 31
  • 16
  • 14
  • 11
  • 7
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
PLRV219

Isotype

IgG2b
  • Host

    • 113
    • 58
    • 2
    Mouse

    Purpose

    Mouse IgG2b Isotype Control Purified

    Specificity

    This mouse IgG2b monoclonal antibody (clone PLRV219) reacts with undefined epitope on a plant pathogen.

    No Cross-Reactivity

    Human, Mouse, Pig, Rat

    Purification

    Purified by protein-A affinity chromatography.

    Purity

    > 95 % (by SDS-PAGE)
  • Application Notes

    Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Do not freeze.
  • Target

    IgG2b

    Target Type

    Antibody
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