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Mouse IgM Isotype Control

Name: Mouse IgM Isotype Control
Brand: antibodies-online
SKU: ABIN93881
Availability: InStock

IsoC, FACS, ELISA, WB, IHC (p), IHC (fro), ICC, IP Monoclonal PFR-03 IgM unconjugated

Flow cytometry analysis: Example of nonspecific mouse IgM (PFR-03) PE signal on human peripheral blood, surface staining, 3 μg/mL. (Mouse IgM Isotype Control)

FACS

1 image

Catalog No. ABIN93881

$351.26

Plus shipping costs $50.00

100 μg

Shipping to: United States

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Quick Overview for Mouse IgM Isotype Control (ABIN93881)

Target

IgM

Clonality

Monoclonal

Application

Isotype Control (IsoC), Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone

PFR-03

Isotype

IgM

Host
60

53

38

5

2

2

2

2

2

2

2

2

2

2

1
Mouse

Purpose

Mouse IgM Isotype Control Purified

No Cross-Reactivity

Human, Mouse, Pig, Rat

Characteristics

This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.

Purification

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity

> 95 % (by SDS-PAGE)
Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC

Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated

Mouse IgM isotype control (PE)
FACS, ELISA Monoclonal 11E10 IgM PE

Rabbit IgM isotype control
IsoC, ELISA, IHC Polyclonal IgM unconjugated

Rat IgM isotype control (PE)
FACS, ELISA Monoclonal NIP-M-2 IgM PE

Mouse IgM isotype control (APC)
FACS, ELISA Monoclonal 11E10 IgM APC

Mouse IgM isotype control (Biotin)
FACS, ELISA Monoclonal 11E10 IgM Biotin

Mouse IgM isotype control (SPRD)
FACS, ELISA Monoclonal 11E10 IgM SPRD

Mouse IgM isotype control (PE-Cy7)
FACS, ELISA Monoclonal 11E10 IgM PE-Cy7

Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated

Application Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Target

IgM

Target Type

Antibody

Gene ID

35