Chicken IgG Isotype Control
Quick Overview for Chicken IgG Isotype Control (ABIN964209)
Target
Fragment
Application
Isotype
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Host
- Chicken
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Supplier Product No.
- 003-0103
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Supplier
- Rockland
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Purpose
- Chicken IgG Fc Fragment
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, anti-Chicken IgG and anti-Chicken IgG F(c). No reaction was observed against anti-Chicken IgG F(ab')2 or anti-Papain.
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Characteristics
- Chicken IgG F(c) fragment, Chicken IgG Fc fragment, Chicken IgY F(c) fragment, Chicken IgY Fc fragment
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Purification
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.
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Sterility
- Sterile filtered
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Application Notes
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Application Note: Chicken IgG F(c) fragment has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to opening. Stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- IgG
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Target Type
- Antibody
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Background
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This antibody possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
Target
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