Chicken IgG isotype control (FITC)
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- Target See all IgG products
- IgG
- Fragment
- Fc fragment
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Host
- Chicken
- Conjugate
- FITC
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 003-0203
- Supplier
- Rockland
- Purpose
- Chicken IgG Fc Fragment Fluorescein Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken IgG, anti-Chicken IgG F(c) and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(ab')2 or anti-Papain.
- Characteristics
- Chicken IgG F(c) fragment fluorescein conjugated, Chicken IgG F(c) fragment FITC conjugated, Chicken IgY F(c) fragment fluorescein conjugated, Chicken IgY F(c) fragment FITC conjugated
- Purification
- This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
- Isotype
- IgG
- Labeling Ratio
- 1.0
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- Application Notes
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Application Note: CHICKEN IgG F(c) fragment Fluorescein conjugated has been tested by SDS-PAGE and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This antibody possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
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