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Chicken IgG isotype control (HRP)

SDS IgG HRP
Rockland
Catalog No. ABIN964215
Supplier Product No.: 003-0302

Quick Overview for Chicken IgG isotype control (HRP) (ABIN964215)

Target

IgG

Conjugate

HRP

Application

SDS-PAGE (SDS)

Isotype

IgG
  • Host

    • 133
    • 126
    • 124
    • 85
    • 82
    • 60
    • 57
    • 55
    • 46
    • 42
    • 38
    • 33
    • 32
    • 32
    • 24
    • 18
    • 15
    • 9
    • 8
    • 7
    • 3
    • 2
    Chicken

    Supplier Product No.

    003-0302

    Supplier

    Rockland

    Purpose

    Chicken IgG Whole Molecule Peroxidase Conjugated

    Cross-Reactivity (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Chicken IgG and anti-Chicken Serum.

    Characteristics

    Chicken IgG peroxidase conjugated, Chicken IgG HRP conjugated, Chicken IgY peroxidase conjugated, Chicken IgY HRP conjugated

    Purification

    This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. 
  • Application Notes

    Application Note: Chicken IgG whole molecule peroxidase conjugation has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 1.0 mL

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: None

    Preservative

    Without preservative

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Expiry Date

    12 months
  • Target

    IgG

    Target Type

    Antibody

    Background

    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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